Coding

Part:BBa_K3657019:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 21:19, 27 October 2020 by Liza ch (Talk | contribs) (→‎Design Notes)

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Ribonuclease A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The aminoacid sequence was obtained from the Uniprot database (P61823). The nucletide sequence was then generated and codon optimised for E. coli.

Source

Bos taurus

References

delCardayré SB, Ribó M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT. Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 1995 Mar;8(3):261-73. doi: 10.1093/protein/8.3.261. PMID: 7479688.

This part on Uniprot: https://www.uniprot.org/uniprot/P61823