Reporter

Part:BBa_K121015

Designed by: Masashi Kajita, Ryoji Sekine   Group: iGEM08_Tokyo_Tech   (2008-10-18)
Revision as of 03:48, 30 October 2008 by Ryo (Talk | contribs) (→‎Pressure response)

pL GFP on pSB6

pL (lambda cI regulated) + RBS + GFP + TT on pSB6 (Part:BBa_I739201).

Usage and Biology

This biobrick is designed for [http://2008.igem.org/Team:Tokyo_Tech iGEM2008 Tokyo_Tech] as a weakly pressure inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 125
    Illegal BamHI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 812

Pressure response

figure. Pressure response of PL. PL activity increased about 1.4-fold under 30 MPa. ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here!!]


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Categories
Parameters
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