Coding

Part:BBa_K3610006

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-30)
Revision as of 20:21, 27 October 2020 by Jtrott (Talk | contribs) (→‎Usage and Biology)

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EFR from A. thaliana - without signal sequence

This part is the EFR receptor from A. thaliana. It is similar to Part:BBa K3610005 but it lacks the signal sequence and is therefore not in frame. It needs to be expressed with a signal sequence. Either with the native signal sequence or with a signal sequence from another organism.

Usage and Biology

Elongation factor-thermo unstable receptor (EFR) from A. thaliana is a plant pattern-recognition receptor (PRR). It is a cell surface receptor and part of the plants firts defence mechanism against potential pathogens. The EFR receptor is also a leucin-rich-repeats (LRR) receptor-like serine/threonine-protein kinase. The protein consists of an extracellular domain with leucin-rich repeats, a ligand binding domain found in many receptors, a single-pass transmembrane domain and finally an intracellular kinase domain. The ligand binding domain from EFR has high specificity to a bacterial pathogen-associated moleculat pattern (PAMP), namely the epitope elf18 of the abundant protein Elongation Factor Tu (EF-Tu), which is catalyzes the binding of aminoacyl-tRNA (aa-tRNA) to the ribosome in most prokaryotes and therefore is evolutionarily highly conserved. This makes the EFR a receptor that can be activated by the presence of a huge variety of bacteria. Upon binding of the ligand to the extracellular domain, the receptor dimerizes with its coreceptor BRI1-associated receptor kinase (BAK1). This interaction triggers the activation of the intracellular kinase domain of EFR and BAK1, initiating a signal cascade leading to an upregulation of immune response mechanisms.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2683
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2683
    Illegal BglII site found at 2348
    Illegal BglII site found at 2376
    Illegal BglII site found at 2919
    Illegal XhoI site found at 2372
    Illegal XhoI site found at 2962
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
    Illegal AgeI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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