Composite

Part:BBa_K3654015

Designed by: Hyun Soo Kang   Group: iGEM20_KSA_KOREA   (2020-10-27)
Revision as of 20:08, 27 October 2020 by Registry (Talk | contribs)

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PlacI-RBS-mprA-T7 terminator - PT7-LacO-RBS-CouP-T7 terminator

This composite part is composed of two composite parts. One is the PlacI-RBS-mprA-T7 terminator (BBa_K3654012), which is responsible for the costitutively expression of mprA, and the other is the PT7-LacO-RBS-CouP-T7 terminator (BBa_K3654014), which is responsible for the IPTG inducible expression of CouP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 115
    Illegal PstI site found at 1087
    Illegal PstI site found at 1525
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1087
    Illegal PstI site found at 1525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1670
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 115
    Illegal PstI site found at 1087
    Illegal PstI site found at 1525
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 115
    Illegal PstI site found at 1087
    Illegal PstI site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1428


[edit]
Categories
Parameters
None