Part:BBa_K143062

LacI repressor protein - Terminator

LacI transcriptional repressor protein (BBa_K143033) coupled to the double terminator (BBa_B0015).

The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis. LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.

Please note. This part has picked up a mutation, which changes an amino acid residue from a Serine to a Leucine but does not cause a frame shift. Given the size of the protein, this should not adversley afect its function to a significant degree.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]