Regulatory

Part:BBa_K3505016

Designed by: Asteria Tsapadikou,Emmanouil Stylianakis   Group: iGEM20_Thessaly   (2020-10-20)
Revision as of 18:40, 27 October 2020 by Asteriat (Talk | contribs) (→‎Design Notes)


araC-ParaBAD:RBS GB compatible with A1-B1

long

Usage and Biology

The PBAD promoter, derived from the arabinose operon andis an inducible promoter for arabinose. The use of an inducible promoter provides a most reversible and flexible gene circuit and at the same time exhibits a higher efficiency and lower side effects as cell death. The araC gene, is a regulatory gene and is located upstream of the L-arabinose operon, encodes a positive regulatory protein AraC, required for L-arabinose utilization in Escherichia coli. The araC gene has a constitutive promoter.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 BBa_K3505007

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