Composite

Part:BBa_K3621015

Designed by: Ethan Levy   Group: iGEM20_MIT   (2020-10-27)
Revision as of 18:33, 27 October 2020 by Levyethan (Talk | contribs)

__NOTOC__ BBa_K3621015 short Contents: NF-kB Responsive Promoter, Csy4, SynthpA What is it?: A Composite part; it is a genetic circuit with NF-kB responsive promoter and Csy4 gene allowing for Csy4 expression when NF-kB is present What does it do?: It's responsible for the expression of the endoribonuclease, Csy4 in the presence of NF-kB. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism. How to use it?: This part was used in our Immunomodulation System and computational model. See more on the MIT iGEM 2020 wiki: https://2020.igem.org/Team:MIT link text

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1284
    Illegal PstI site found at 1201
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1201
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1284
    Illegal PstI site found at 1201
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1284
    Illegal PstI site found at 1201
    Illegal AgeI site found at 1290
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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