Part:BBa_K3450004

Pdx1: A transcription factor required for embryonic development of pancreas

Pancreas/ duodenum homeobox protein 1 (PDX1) or insulin promoter factor 1 is a homeodomain transcription factor. It is involved in transcriptional activation of insulin, somatostatin, islet amyloid polypeptide, glucokinase, and type-2 glucose transporter genes. It is required for early embryonic development of the pancreas and proximal duodenum. It plays an important role in the specification of different endocrine lineages, and maintenance of beta-cell function.[1] Transient expression of PDX1, NEUROG3, and/or MAFA has been successfully used to reprogram intestinal crypt cells into glucose-responsive insulin-producing neoislet cells.[2]

References

[1] Zhu, Y., Liu, Q., Zhou, Z. & Ikeda, Y. PDX1 , Neurogenin-3 , and MAFA : critical transcription regulators for beta cell development and regeneration. 1–7 (2017). doi:10.1186/s13287-017-0694-z https://stemcellres.biomedcentral.com/track/pdf/10.1186/s13287-017-0694-z

[2] Chen YJ, Finkbeiner SR, Weinblatt D, Emmett MJ, Tameire F, Yousefi M, Yang C, Maehr R, Zhou Q, Shemer R, Dor Y, Li C, Spence JR, Stanger BZ. De novo formation of insulin-producing "neo-β cell islets" from intestinal crypts. Cell Rep. 2014 Mar 27;6(6):1046-1058. doi: 10.1016/j.celrep.2014.02.013. Epub 2014 Mar 6. PMID: 24613355; PMCID: PMC4245054. https://pubmed.ncbi.nlm.nih.gov/24613355/

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 705
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 169
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 258
  Illegal NgoMIV site found at 285
  Illegal NgoMIV site found at 747
  
• 1000
  COMPATIBLE WITH RFC[1000]