Device

Part:BBa_K3629015

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-17)
Revision as of 17:44, 27 October 2020 by Sravyakakumanu (Talk | contribs)


Nourseothricin resistance expression construct with gibson homology sequences

Usage and Biology

Design

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1590
    Illegal EcoRI site found at 1449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1449
    Illegal NheI site found at 1584
    Illegal SpeI site found at 1591
    Illegal PstI site found at 1605
    Illegal NotI site found at 7
    Illegal NotI site found at 1598
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1449
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1591
    Illegal EcoRI site found at 1449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1449
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1591
    Illegal PstI site found at 1605
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None