Composite

Part:BBa_K3424023:Design

Designed by: Julie Mallinger   Group: iGEM20_UFlorida   (2020-10-27)
Revision as of 17:43, 27 October 2020 by DavidMurcia (Talk | contribs)

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pTet-SCRIBE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1879
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1284
    Illegal XhoI site found at 518
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1879
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Part:BBa_K3086001

https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf?fbclid=IwAR22C9bYtULbPECeq8TFaAhzM7D4_RDMQ9SoeSoqnM6OdHqtg_-hpK6GoXE


References

Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3