Signalling

Part:BBa_K3482005

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-19)
Revision as of 17:42, 27 October 2020 by Jeremy95 (Talk | contribs) (→‎Usage and Biology)

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NSP4 secretion signal peptide

This part codes for a signal peptide, which can be fused to the N-terminus of a polypeptide in order to export the newly synthesised protein out of the cell.

Usage and Biology

The signal peptide 4 (NSP4) was selected because it should improve the secretion of a targeted protein. This peptide is part of the Sec-dependent secretion pathway which is the main secretion pathway in E. coli. This NSP4 peptide fused to the N-terminus of a recombinant protein promotes its translocation across the plasma membrane. This peptide was choosen by our team to enhance azurin export in our E. coli Nissle 1917 strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None