Intermediate

Part:BBa_K133011:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)
Revision as of 01:42, 30 October 2008 by Ancksha (Talk | contribs)

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CF213-UreB-linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 877
    Illegal AgeI site found at 2055
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 763


Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).


Source

References