Tag

Part:BBa_K3550008:Design

Designed by: Jiehui Ye   Group: iGEM20_FAFU-CHINA   (2020-10-27)
Revision as of 16:59, 27 October 2020 by Nohara (Talk | contribs) (→‎References)


Removable protein purification tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The upstream of the tag needs to use linker

Source

References

Anandharaj M, Lin YJ, Rani RP, Nadendla EK, Ho MC, Huang CC, Cheng JF, Chang JJ, Li WH. Constructing a yeast to express the largest cellulosome complex on the cell surface. Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2385-2394. doi: 10.1073/pnas.1916529117. Epub 2020 Jan 17. PMID: 31953261; PMCID: PMC7007581.