RNA

Part:BBa_K3406015

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-10-27)
Revision as of 16:50, 27 October 2020 by Sorrow-filter (Talk | contribs)

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LwaCas13a-SARS-CoV-crRNA

The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to LwaCas13a and SARS-CoV, which could combine with LwaCas13a protein and guide it to target SARS-CoV sequence, thus activating the collateral cleavage of LwaCas13a protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None