Composite

Part:BBa_K3484001

Designed by: Eduard Sune & Andreu Pascuet   Group: iGEM20_UPF_Barcelona   (2020-10-26)
Revision as of 16:43, 27 October 2020 by Andreupf99 (Talk | contribs)

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Intein-mediated T3(THRB) → sfGFP (transcriptional unit)

This part is the transcriptional unit for BBa_K3484000. This part expresses constitutively an intein-mediated T3 biosensor. It is conformed by the Mycobacterium tuberculosis (Mtu) RecA intein, but replacing its endonuclease domain by the thyroid hormone receptor β (THR-β) ligand binding domain (LBD). The extein (the side proteins that the intein will splice and join) is conformed by a split super-folder GFP (sfGFP). In presence of T3, the intein domains splice the protein and full sfGFP is generated.

To see the full information and characterization, take a look at the BBa_K3484000 protein domain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 235
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 722
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 278
    Illegal SapI.rc site found at 1637


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
None