Composite
Part:BBa_K3595017
Designed by: Ying Li Group: iGEM20_GZ_HFI (2020-10-27)
GPPS-MS regulated by pTac
use pTac promotor (BBa_K3595003),strong RBS (BBa_B0034) , RiboJ(BBa_K2615996), coding sequence GPPS(BBa_K3595016), coding sequence MS(BBa_K3595015) and terminator(BBa_B0010) ,will generate GGP and MS in case of IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2573
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2438
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 741
Illegal AgeI site found at 1919 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |