Device

Part:BBa_K3402050

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-11)
Revision as of 16:14, 27 October 2020 by ZZ 0991 (Talk | contribs)


report circuit

This device is composed of promoter Ptef1 (BBa_K3402007), reporter gene yeGFP (BBa_K3402000), terminator Tsyn7 (BBa_K3402001).
Report circuit-zzh.png

Usage and Biology

The green fluorescent protein (yeGFP) gene can be used as reporter gene and express in Starmerella bombocola. We conducted this device to test if the yeGFP can express in Starmerella bombocola, so we connected this device. Besides, the hygromycin resistance gene (hph) was built into the plasmid as the marker gene to determine if the transformation is successful. Two homologous arms are used to intsert this gene into PXA1 site.
As a result, the yeGFP was succefully expressed in Starmerella bombocola. Finally, we can link different promoters to yeGFP to know the strength of promoters by testing different fluorescence intensity and transcription levels analysis of different strains.

a) Fluorescence intensity of different promoters. b) Transcription levels of different promoters. c) GFP was successfully expressed in cells

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1051
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1492


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Categories
Parameters
None