Composite

Part:BBa_K3365016:Design

Designed by: JUNYAN LI   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-23)
Revision as of 15:34, 27 October 2020 by Little ghost (Talk | contribs) (→‎Design Notes)


pBAD upstream of eGFP with inhibition unit containing lure2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1053
    Illegal XhoI site found at 1062
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


Design Notes

The restriction cutting sites are added to the fragment during PCR for easy ligation to the backbone.

Source

transcription inhibition

References