Part:BBa_K133001

CF-multiHP-RGD-Histop

Chimeric flagellin with added multiepitope at the C-terminal. Chimeric fusion protein retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production. We also designed a highly immunogenic fusion protein - multiepitope - which combines most immunogenic portions of three essensial H. pylori proteins: VacA, UreB, HpaA.

Since the protein was designed to be produced, we additionally added RGD-Hisstop tag (for the part description and implication follow: [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1207
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 764
  Illegal BamHI site found at 1275
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2091