Coding

Part:BBa_K3468025

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-18)
Revision as of 15:06, 27 October 2020 by Seesky (Talk | contribs)

PETase S46D


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This mutation increases the hydrogen bond near S46. The hydrogen bond is an interaction formed by N, O and H in a protein, which can stabilize the structure of the protein and improve thermal stability.

We use FoldX to judge the thermal stability of S46D and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out what interaction make it become stable.

Fig.1 PETase A40P,S54W,T113P,T116R,Q119D,Q127L,Q182I,S193R,S214F,K252L,R260F,N275P in docking
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Parameters
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