Reporter

Part:BBa_K133055:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)
Revision as of 01:01, 30 October 2008 by Ancksha (Talk | contribs) (→‎Source)

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mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

Red Flourescence Protein was obtaind from previousely designed biobrick of 2007 iGEM team Ljubljana containing Red Flourescence Protein with added nuclear localisation signal and mutated PstI restriction site.

References