Part:BBa_K3407017:Design
Cry7Ca1 toxin with inducible T7 promoter, RBS and T7 terminator
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 688
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1388
Illegal XhoI site found at 367 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa (Figure 2). The Cry7Ca1 toxin (activated Cry7Ca1 protein) crystal structure has been published and can be found in the Protein Data Bank under the accession number 5ZI1 [1].
The Cry7Ca1 toxin gene was codon-optimized for Escherichia coli using the GenSmartTM Codon Optimization Tool (https://www.genscript.com/tools/gensmart-codon-optimization). The forbidden restriction enzymes sites are the following: - BioBrick forbidden restriction sites: EcoRI, XbaI, SpeI, PstI - Type IIS forbidden restriction sites: BsaI, SapI and Bpil
Source
Synthetically synthesised.
References