Coding

Part:BBa_K3468072

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 13:59, 27 October 2020 by Dtvnhy (Talk | contribs)

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PETase S188N

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from S to N at 188 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Adding hydrogen bonds to stabilize α5-β7loop

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening. it formed hydrogen bonds with 186-HIS and 190-ASN. after mutation to ASN, it formed hydrogen bonds with 190-ASN, which stabilized α5-β7loop and was beneficial to improve the thermal stability of PETase.

Fig.1 S188N in PyMOL


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