Composite

Part:BBa_K3628024

Designed by: Tianzuo Zeng   Group: iGEM20_SMS_Shenzhen   (2020-10-27)
Revision as of 13:34, 27 October 2020 by Registry (Talk | contribs)

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J23106-RBS-T7 RNA polymerase N 1~564-Vvd-RBS-Vvd-T7 RNA polymerase C 565~883

This part is composed of photoswitches linked to split T7RP. Two coding parts are linked by a spacer. A promoter, J23106, is placed at the start.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1953
    Illegal AgeI site found at 2181
    Illegal AgeI site found at 2498
    Illegal AgeI site found at 2726
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None