Intermediate

Part:BBa_K133056:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)
Revision as of 00:36, 30 October 2008 by Ancksha (Talk | contribs) (→‎Source)

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HpaA-LPP-RGD-Histop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 170
    Illegal BglII site found at 266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 877


Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).


Source

GeneArt (LPP, primers for RGD-Histop annealing), HpaA was obtained with PCR from genomic DNA from H.pylori.

References