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Part:BBa_K3365059

Designed by: Zou Aixuan   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-22)
Revision as of 12:45, 27 October 2020 by Gyaxxxxxx (Talk | contribs)


kanamycin resistant gene with homologous arms

This part is designed to knock out genome rpoZ, which codes for the Omega submit of RNA polymerase. We added 40 base pairs homologous arm at both end of Kanamycin resistant gene in order to replace rpoZ.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None