Composite

Part:BBa_K133050

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)
Revision as of 00:19, 30 October 2008 by Ancksha (Talk | contribs)

TetR-CF-RGD-Histop

TetR repressible promoter with ribosomal binding site.

Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1267
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 824
    Illegal BamHI site found at 1335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1618


[edit]
Categories
Parameters
None