RNA

Part:BBa_K3328035:Experience

Designed by: Chaoxin Chen   Group: iGEM20_OUC-China   (2020-10-04)
Revision as of 11:47, 27 October 2020 by Txzz (Talk | contribs) (→‎Applications of BBa_K3328035)

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The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.


Applications of BBa_K3328035

800px-T--OUC-China--part_XOR2.jpg

The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).

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