Composite

Part:BBa_K3407018

Designed by: Javier Navarro Delgado   Group: iGEM20_TUDelft   (2020-10-23)
Revision as of 11:16, 27 October 2020 by Javier (Talk | contribs)


Mini-3 endoribonuclease with araBAD promoter - RBS - and rrnBT1 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 300
    Illegal PstI site found at 527
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 300
    Illegal BglII site found at 309
    Illegal BamHI site found at 809
    Illegal XhoI site found at 818
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56

Usage and Biology

This part was used to Express mini-3 from B. subtilis (BBa_K3407002)

[edit]
Categories
Parameters
None