![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3407018
Designed by: Javier Navarro Delgado Group: iGEM20_TUDelft (2020-10-23)
Mini-3 endoribonuclease with araBAD promoter - RBS - and rrnBT1 terminator
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 300
Illegal XbaI site found at 673
Illegal PstI site found at 527 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 300
Illegal PstI site found at 527 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 300
Illegal BglII site found at 309
Illegal BamHI site found at 809
Illegal XhoI site found at 818 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 300
Illegal XbaI site found at 673
Illegal PstI site found at 527 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 300
Illegal XbaI site found at 673
Illegal PstI site found at 527
Illegal AgeI site found at 74 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 56
Usage and Biology
This part was used to Express mini-3 from B. subtilis (BBa_K3407002)
[edit]
Categories
Parameters
None |