Coding

Part:BBa_K3331002

Designed by: Wang Weizhe   Group: iGEM20_XJTU-China   (2020-10-20)
Revision as of 10:47, 27 October 2020 by Cycling (Talk | contribs)


E.coli pgmA

pgmA [Escherichia coli (strain K12)] Encodes 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase that catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate. This part can catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 1770bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.
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