Coding

Part:BBa_K3331001

Designed by: Wang Weizhe   Group: iGEM20_XJTU-China   (2020-10-20)
Revision as of 10:45, 27 October 2020 by Cycling (Talk | contribs)


E.coli galU

galU [Escherichia coli str. K-12 substr. MG1655] Encodes Glucose-1-phosphate uridylyltransferase, or UGPase, which catalyzes the production of UDP-glucose from glucose-1-phosphate and UTP. .This sequence comes from the genome of E.coli and we optimized the codon of this DNA sequence so that it could be expressed more efficiently in Bacillus subtilis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 981bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.
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