Part:BBa_K3329018
Streptococcal Collagen-like 2 without V domain optimized V1
This sequence code for Streptococcal Collagen-like protein, which is used to modify the bacteria cellulose.Such protein is capable of recreating the texture of leather.
The wild type of this protein usually contains a V domain which is responsible for stabilizing the downstream folding process of scl2 protein. However, in our project, we designed a SpyTag003-scl2 with V domain-SpyTag003-scl2 without V domain-SpyTag003 structure, in which the V domain in first scl2 protein can stabilize the folding of the other scl2 downstream it. Therefore, the scl2 protein in our project has two different versions from the perspective of V domain. Moreover, due to the different codon preference of P. Pastoris and E. coli, the sequence of scl2 should also be divided into two different types — one for P. Pastoris, and one for E. coli. Together, considering these two factors, we generated scl2 proteins with 4 different types and 2 different versions. The first two don't contain V domain, and the second two contain V domain. Also, the first version (V1) refers to the codon optimization for P. Pastoris, and V2 refers to the codon optimization for E. coli.
We also built a model calculating the optimized ratio of SpyCatcher003 to SpyTag003 for gel formation. See more details in our Engineering Success webpage: https://2020.igem.org/Team:BNDS_China/Engineering
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 292
Illegal NgoMIV site found at 324
Illegal AgeI site found at 121 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 217
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