RNA

Part:BBa_K3328010:Design

Designed by: Chaoxin Chen   Group: iGEM20_OUC-China   (2020-10-03)
Revision as of 09:17, 27 October 2020 by Txzz (Talk | contribs) (→‎Design Notes)


trigger of toehold


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

800px-T--OUC-China--design_fig2.png.jpeg

Toehold switch is composed of cis-acting element RNA hairpins and trans-acting factor trigger RNA. The binding of a trigger RNA to the toehold sequence allows for a branch migration process, exposing AUG and RBS for translation initiation.

Source

synthesize from company

References