Coding

Part:BBa_K3329022

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-10)
Revision as of 09:05, 27 October 2020 by CJHH (Talk | contribs)

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CBM optimized V1

The cellulose binding module (CBM) help connect cellulose and Spycatcher003.

It's noteworthy that, in our Protein Crosslinking design, we intended to use double CBM in order to anchor the CBM and SpyCatcher003 firmly. Moreover, CBM would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same CBM sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from CBM optimized V1 to CBM optimized V4, in which the CBM optimized V1 and V2 are for P. Pastoris, and CBM optimized V3-4 are for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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