Composite
Part:BBa_K3447108
Designed by: Yushan Geng, Yu Ma Group: iGEM20_Jilin_China (2020-10-27)
Expression of kanamycin resistance
This is a part with kanamycin resistance gene (BBa_K3447003).
Usage and Biology
It is well-known that kanamycin has the ability to inhibit the growth of bacteria, so we expect that with the expression of KanR gene, our engineered bacteria would be able to survive under the attack of kanamycin.
Characterization
To verify the construction of KanR, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1A). Compared with the control group, bacteria constructed with KanR show more than 10 times the resistance to kanamycin (Fig. 1B). Zone of inhibition test also shows that our KanR works well with 0.1% kanamycin added (Fig. 1C).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 26
Illegal NheI site found at 49 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 110
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1039
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Categories
Parameters
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