Reporter

Part:BBa_K934025

Designed by: Toshiki Hashimoto   Group: iGEM12_Tokyo_Tech   (2012-09-17)
Revision as of 08:50, 27 October 2020 by Kanta (Talk | contribs) (→‎Contribution: Waseda 2020)

Plux/tet-GFP

We constructed this part by ligating Plux/tet hybrid promoter (BBa_K934024) to the upstream of promoterless GFP generator (BBa_I13504).

This work was done by M.M, X.T and S.L.

To characterize the function of the Lux/Tet hybrid promoter(BBa_934024), we constructed this part Plux/tet-GFP (BBa_ K934025) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-Ptrc-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline).

In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.

We improved previous Plux/tet hybrid promoter (BBa_K176000).


For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

Contribution and improvement: Waseda 2020

By addition of LVA degradation tag, Waseda2020 improved this part in terms of quick response of genetic circuit
See BBa_K3580003

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 751


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Categories
Parameters
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