Coding

Part:BBa_K3329012

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-05)
Revision as of 08:47, 27 October 2020 by CJHH (Talk | contribs)


SpyCatcher003 optimized V1

This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyTag003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyCatcher003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyCatcher003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 12 versions of CDS is documented from SpyCatcher003 optimized V1 to SpyCatcher003 optimized V12, in which the SpyCatcher003 optimized V1-V6 are for P. Pastoris, and SpyCatcher003 optimized V7-V12 are for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None