Part:BBa_R0071

Promoter (RhlR & C4-HSL regulated)

Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.

This is the natural sequence taken from Pseudomonas aeruginosa.

Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.

Usage and Biology

RhlR binds to the promoter region and activates transcription when in the presence of its autoinducer -- N-butyryl-homoserine lactone (also known as C4-HSL), which is produced by RhlI.

From [Medina03]: ... RhlR binds to this DNA sequence, both in vivo and in vitro, in the presence and in the absence of C4-HSL. The binding of RhlR(C4-HSL) to the las box is a necessary condition for rhlAB expression while the binding of RhlR alone to the same DNA sequence seems to repress rhlAB transcription.

From [Medina03]: The LasR(3-O-C12-HSL) complex can activate rhlAB promoter expression but at a considerably lower level than the RhlR(C4-HSL) complex.

From the data in [Medina03], LasR activates rhlAB at 7.8% of the activation level achieved by RhlR.

From the data in [Pearson97], the activation by LasR is about 3% of that by RhlR.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

biology	-NA-
direction	Forward
negative_regulators	-NA-
positive_regulators	1