RNA

Part:BBa_K3645016

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-27)
Revision as of 07:17, 27 October 2020 by Teddy Huang (Talk | contribs)

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sgRNA scaffold

Template of sgRNA. Use Golden Gate (BsaⅠ) to replace the template sequence with a ~20bp guide sequence. Add 'tagc' on the 5'-terminal of your guide sequence and 'aaac' on the reverse 5'-terminal.

5'-tagc-20bp-****-3'
3'-****-20bp-caaa-5'

Transcription product is a single strain sgRNA for dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 135
    Illegal BsaI.rc site found at 37


[edit]
Categories
Parameters
None