Coding

Part:BBa_K3447000:Design

Designed by: Jiahao Yu, Xinyue Zhang, Yu Ma   Group: iGEM20_Jilin_China   (2020-09-03)
Revision as of 06:56, 27 October 2020 by Alswey (Talk | contribs) (→‎References)


luxI, an autoinducer synthetase for AHL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a repetitive sequence between cvaA and cvaB gene. In order not to increase restriction sites, we modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.

Source

We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164
We ordered the part DNA from a synthesis company.

References