Coding

Part:BBa_K3406002

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-08-26)
Revision as of 06:29, 27 October 2020 by Sorrow-filter (Talk | contribs)

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PsmCas13b coding sequence

The coding sequence of PsmCas13a protein.It contains rare codons, so it needs to be expressed in E. coli strains which is rich in corresponding tRNAs like E. coli Rosetta2(DE3)pLysS.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 779
    Illegal BglII site found at 3113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 1135
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 393
    Illegal SapI.rc site found at 2124
[edit]
Categories
Parameters
None