Coding

Part:BBa_K3406001

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-08-16)
Revision as of 06:26, 27 October 2020 by Sorrow-filter (Talk | contribs)

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CcaCas13b coding sequence

The original CcaCas13b protein gene contains rare codons. We optimized the codon to make it could be expressed in common E. coli protein expression strains, such as E. coli BL21(DE3).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 954
    Illegal AgeI site found at 1806
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None