Coding

Part:BBa_K3645009:Design

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-26)
Revision as of 03:24, 27 October 2020 by Teddy Huang (Talk | contribs) (→‎Design Notes)

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PmCDA from Target-AID


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 671
    Illegal XhoI site found at 280
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a fragment of original plasmid design, and contains other auxiliary parts (e.g. linkers, etc.). UGI is included so no extra ligation of UGI is needed

Source

Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A. Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Apr;3(4):423-429. doi: 10.1038/s41564-017-0102-6. Epub 2018 Feb 5. PMID: 29403014.

References