Part:BBa_K3610030:Design

BAK1/YFP

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1560
  Illegal PstI site found at 650
  Illegal PstI site found at 695
  Illegal PstI site found at 985
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1560
  Illegal PstI site found at 650
  Illegal PstI site found at 695
  Illegal PstI site found at 985
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1560
  Illegal PstI site found at 650
  Illegal PstI site found at 695
  Illegal PstI site found at 985
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1560
  Illegal PstI site found at 650
  Illegal PstI site found at 695
  Illegal PstI site found at 985
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part contains the orignial signal peptide sequence. It therefore has a start codon. We were able to sequence this part, which confirmed that the sequence we used was actually the correct one.

The 15 amino acid linker was chosen because it consists of small amino acids (all Ser and Gly). There is no additional tag for Western Blotting as stains for YFP are widely available.

Source

Composed of the basic parts: BBa_K319005, BBa_K3610001, BBa_K416001, BBa_J176006, BBa_K2753050

References

Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.

Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.

Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.