Coding

Part:BBa_K3595005

Designed by: Ying Li   Group: iGEM20_GZ_HFI   (2020-10-26)
Revision as of 02:14, 27 October 2020 by Registry (Talk | contribs)

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Mutant cysE-256

cysE-256 is a mutant of cysE gene, which is obtained by substituting the ATG (Met) on the 256th position into TGG (Trp).In the pathway of converting hydrogen sulfide to L-cysteine, L-serine and Acetyl-CoA form O-acetylserine catalyzed by L-serine O-acetyltransferase (SAT). SAT is encoded by gene cysE, and its feedback is inhibited by the final product L-cysteine . In order to induce overproduction of L-cysteine, we need to develop a mutant gene of cysE which will code for feedback inhibition-insensitive SAT.We tried to construct different mutants through site mutation, and cysE-256 is one of the different mutants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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