Regulatory

Part:BBa_K3411030

Designed by: Janet Standeven, Nikhita Lalwani, Sindhura Mettupalli, Kathy Ye, Sachintha Ashok, Saif Khan   Group: iGEM20_Lambert_GA   (2020-10-23)
Revision as of 01:15, 27 October 2020 by Msindhu01824 (Talk | contribs)


PdcuS

To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate with nitrate levels to a reporter protein. Thus, we needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice used the PdcuS protomer, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate.

Due to a change in iGEM Parts Registry Criteria, the Lambert_GA 2020 team created biobricks Part:BBa_K3411030 (for the Pdcus promoter) because the 2015 HKUST-Rice team was not required to create the basic parts for the corresponding composite part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None