Coding

Part:BBa_K3629008

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 00:10, 27 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Usage and Biology)


Modified Trichoderma reesei EGI with 6X His tag

Temperature and pH optimized endoglucanase I coding sequence from Trichoderma reesei with 6x His tag.

Usage and Biology

IN-SILCO CHARACTERIZATION: MODELLING

Design

The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
//awards/part_collection
//chassis/eukaryote/yeast
Parameters
None