Composite

Part:BBa_K3425070

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-26)
Revision as of 22:26, 26 October 2020 by Tereza H (Talk | contribs)


Modular outer membrane display construct for nanobody specific to HSP biomarker

This composite part serves for display of nanobodies on the outer surface of E. coli. It is designed as a modular system of, in which the nanobody module (in this case BBa_K3425043) can be easily exchanged for nanobody of different specificity. All components are tailored for iGEM Type IIS assembly standard.


BBa_K3425042 is a signal peptide directing the fusion protein to the periplasm.
BBa_K3425043 is the nanobody specific for Mycobacterium tuberculosis biomarker HSP 16.3 (BBa_K3425041)
BBa_K3425044 can be used as an outer membrane display system for E. coli


Part number Name Description
BBa_K3425042 pelB signal peptide
BBa_K3425043 B-F10 HSP Nanobody
BBa_K3425044 ehaA Transmembrane domain


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None