Composite

Part:BBa_K3425070:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-26)
Revision as of 21:54, 26 October 2020 by Tereza H (Talk | contribs) (→‎Design Notes)


Modular outer membrane display construct for nanobody specific to HSP biomarker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of three BioBricks tailored for assembly by iGEM Type IIS standard:
BBa_K3425042
BBa_K3425043
BBa_K3425044

It is a modular system for the exposure of nanobodies on the outer surface of E. coli. In this case, the nanobody BBa_K3425043 is specific for HSP protein of Mycobacterium tuberculosis.

Source

Registry of Standard Biological Parts BBa_K3425042 BBa_K3425043 BBa_K3425044

References