Composite

Part:BBa_K3331009

Designed by: Wang Weizhe   Group: iGEM20_XJTU-China   (2020-10-22)
Revision as of 18:56, 26 October 2020 by Cycling (Talk | contribs) (→‎Overview)


B.S galU+B.S pgmA

The yield of EPS can be significantly increased by overexpressing two key enzymes galU and pgmA at the same time. Reference：Levander Fredrik et al. Applied and environmental microbiology,2002,68(2).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Overview

In order to increase the production of exopolysaccharides (EPSs), we altered the levels of enzymes in the central metabolism. We selected and overexpressed two key enzymes, galU and pgmA at the same time. And we found we successfully enhance the production of EPSs in E.coli.DH5α

Figure 1:Plasmid we have constructed for galU&pgmA validations.
Figure 1:Plasmid we have constructed for galU&pgmA validations.
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